Temporary Immersion System in Plant Tissue Culture: Applications, Technical Factors, and Bottlenecks for Efficient Micropropagation

Murgayanti; Alifiyan Anur; Salwa Septiani Rahayu

doi:10.55173/agriscience.v10i1.217

Authors

Murgayanti Department of Agronomy, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor, Sumedang 45363, West Java, Indonesia
Alifiyan Anur Agrotechnology Study Program, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor, Sumedang 45363, West Java, Indonesia
Salwa Septiani Rahayu Agrotechnology Study Program, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor, Sumedang 45363, West Java, Indonesia

DOI:

https://doi.org/10.55173/agriscience.v10i1.217

Keywords:

Acclimatization, Bioreactor, Micropropagation, Plant Tissue Culture, Temporary Immersion System

Abstract

Temporary Immersion System (TIS) has emerged as an efficient platform for micropropagation and conservation of medicinal and horticultural plants. Periodic immersion can facilitate nutrient uptake and gas exchange, with reported effects on shoot multiplication, rooting, and plantlet quality varying according to plant species and culture protocols. Key technical factors, including immersion frequency, immersion duration, bioreactor type, medium volume, explant density, and plant growth regulators, can substantially influence micropropagation efficiency. TIS offers potential for scalable propagation and labor reduction, although hyperhydricity may remain a challenge depending on system configuration and culture conditions. Applications range from high value horticultural crops to medicinal plants, supporting uniform propagation, germplasm conservation, and nursery scale production. Current bottlenecks include standardization of operational parameters, hyperhydricity management, acclimatization success, and integration of technological innovations such as automation, sensors, and data driven control. Future research should focus on optimizing these parameters for diverse species, developing smart TIS systems, and evaluating long term genetic stability and physiological performance to improve its applicability in commercial propagation and conservation. Overall, TIS represents a promising tool for advancing plant tissue culture efficiency and sustainable propagation strategies.

Author Biographies

Murgayanti, Department of Agronomy, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor, Sumedang 45363, West Java, Indonesia

Department of Agronomy, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor, Sumedang 45363, West Java, Indonesia
Alifiyan Anur, Agrotechnology Study Program, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor, Sumedang 45363, West Java, Indonesia

Agrotechnology Study Program, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor, Sumedang 45363, West Java, Indonesia
Salwa Septiani Rahayu, Agrotechnology Study Program, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor, Sumedang 45363, West Java, Indonesia

Agrotechnology Study Program, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor, Sumedang 45363, West Java, Indonesia

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