Characterization of Mangoes In Cirebon Using The Single Nucleotide Polymorphism Method (Snp)
DOI:
https://doi.org/10.55173/agriscience.v9i2.199Keywords:
mango, genetic characterization, Single Nucleotide Polymorphism (SNP)Abstract
Mango (Mangifera indica L.) is a leading horticultural commodity in Cirebon Regency, boasting a diverse array of local varieties with distinct fruit characteristics. Varietal identification has traditionally been based on morphological characteristics, but this method is often inaccurate due to environmental factors. Therefore, a more stable and objective molecular-based approach is needed, one of which is Single Nucleotide Polymorphism (SNP) analysis. This study aims to characterize the genetic diversity of mangoes in Cirebon using SNP markers as a basis for kinship information and potential variety development. The research methods included leaf sampling from several local Cirebon mango varieties, genomic DNA isolation, DNA amplification using the Polymerase Chain Reaction (PCR) method, and sequencing analysis to identify SNP variations. The SNP data obtained were analyzed to assess genetic diversity and kinship among mango varieties. The results showed genetic differences between local mango varieties that could be clearly distinguished using SNP markers. In conclusion, SNP-based characterization of Cirebon mango is effective for revealing genetic diversity and can serve as a basis for variety identification, preservation of local germplasm, and support for mango breeding and development programs in the Cirebon region.
References
Al., V. et. (2005). Kesesuaian Lahan Untuk Tanaman Mangga (Mangifera indica L.) di Buleleng. 1–7.
Ashgi, F. (2021). Analisis Single Nucleotide Polymorphism Gen Faktor Transkripsi MYB dalam Biosintesis Antosianin Kulit Buah Mangga ( Analysis of Single Nucleotide Polymorphism Gene Transcription Factor MYB in Mango Skin Anthocyanin Biosynthesis ). 2020, 1–10.
Di, A., & Cirebon, K. (2019). Risk identification of mango farming in agro-tourism development in cirebon regency. 21(1), 11–16. https://doi.org/10.24198/sosiohumaniora.v21i1.11062
Hafizah, R. A., Adawiyah, R., Harahap, R. M., Hannum, S., Santoso, P. J., & Utara, S. (2018). Aplikasi Marka SSR Pada Keanekaragaman Genetik Durian ( Durio zibethinus Murr .) Di Kabupaten Deli Serdang ,. 11(1), 49–56.
Kuhn, D. N., Bally, I. S. E., Dillon, N. L., Innes, D., Groh, A. M., Rahaman, J., Ophir, R., Cohen, Y., & Sherman, A. (2017). Genetic map of mango: A tool for mango breeding. Frontiers in Plant Science, 8(April), 1–11. https://doi.org/10.3389/fpls.2017.00577
Kuhn, D. N., Dillon, N., Bally, I., Groh, A., Rahaman, J., Warschefsky, E., Freeman, B., Innes, D., & Chambers, A. H. (2019). Estimation of genetic diversity and relatedness in a mango germplasm collection using SNP markers and a simplified visual analysis method. Scientia Horticulturae, 252(March), 156–168. https://doi.org/10.1016/j.scienta.2019.03.037
Lade, B. D., Patil, A. S., & Paikrao, H. M. (2014). Efficient genomic DNA extraction protocol from medicinal rich Passiflora foetida containing high level of polysaccharide and polyphenol. 1–7.
Liang, Q., Pan, H., He, X., Wang, S., Hou, Y., Xiao, H., Xu, G., Yi, R., Lin, D., & Yang, Z. (2024). Population structure and genetic diversity of mango (Mangifera indica L.) germplasm resources as revealed by single-nucleotide polymorphism markers. Frontiers in Plant Science, 15(July), 1–12. https://doi.org/10.3389/fpls.2024.1328126
Prasetiyono, J. (2015). Keragaman Sebelas Klon Mangga Komersial Indonesia ( Variation of Eleven Clones Indonesian Commercial Mango ). 31–40.
Rijzaani, H., Agency, I., & Tasliah, T. (2015). Analisis Keragaman Genetik 161 Aksesi Mangga Indonesia Menggunakan Analisis Keragaman Genetik 161 Aksesi Mangga Indonesia. August. https://doi.org/10.21082/jbio.v9n3.2013.p125-134
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Agricultural Science
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
