Growth Response of Robusta Coffee (Coffea canephora) Cuttings to Arbuscular Mycorrhizal Fungi Dosage Application in Ultisol

Authors

  • Husna State Polytechnic of Lampung, Bandar Lampung, Lampung 35141, Indonesia
  • Hafiz Luthfi State Polytechnic of Lampung, Bandar Lampung, Lampung 35141, Indonesia
  • Sismita Sari State Polytechnic of Lampung, Bandar Lampung, Lampung 35141, Indonesia
  • AH. Maftuh Hafidh Zuhdi State Polytechnic of Lampung, Bandar Lampung, Lampung 35141, Indonesia

DOI:

https://doi.org/10.55173/agriscience.v9i2.193

Keywords:

arbuscular mycorrhizal fungi, Coffea canephora, phosphorus mobilization, Ultisol soil, vegetative propagation

Abstract

Coffee production in Lampung Province faces significant challenges due to the dominance of Ultisol soils characterized by low fertility, high acidity, and phosphorus fixation. This study aimed to determine the optimal dosage of arbuscular mycorrhizal fungi (AMF) inoculum to enhance the growth of robusta coffee (Coffea canephora) cuttings in Ultisol growing media. The experiment was conducted at the State Polytechnic of Lampung from August to October 2025 using a Randomized Complete Block Design (RCBD) with four treatments: M0 (control without mycorrhizal), M1 (10 g mycorrhizal inoculum per plant), M2 (20 g per plant), and M3 (30 g per plant). Each treatment was replicated four times with three plants per experimental unit. Growth parameters measured included leaf number, plant height, and root volume at 0, 3, 9, and 12 weeks after transplanting. Results showed that the M1 treatment consistently produced superior growth performance across all parameters. At 12 weeks after transplanting, M1 achieved 21.44 leaves (53.1% increase), 38.69 cm plant height (48.7% increase), and 44.67 cm³ root volume (108.3% increase) compared to the control. Higher dosages (M2 and M3) showed diminishing returns, indicating excessive inoculum application may cause unfavorable carbon allocation dynamics. This study concludes that 10 g mycorrhizal inoculum per plant is the optimal dosage for enhancing robusta coffee cutting growth in Ultisol nursery systems.

References

Balemi, T., & Negisho, K. (2012). Management of soil phosphorus and plant adaptation mechanisms to phosphorus stress for sustainable crop production: A review. Journal of Soil Science and Plant Nutrition, 12(3), 547-562.

Begum, N., Qin, C., Ahanger, M.A., Raza, S., Khan, M.I., Ashraf, M., Ahmed, N., & Zhang, L. (2019). Role of arbuscular mycorrhizal fungi in plant growth regulation: Implications in abiotic stress tolerance. Frontiers in Plant Science, 10, 1068.

BPS (Badan Pusat Statistik). (2022). Statistik Kopi Indonesia 2022. Jakarta: Badan Pusat Statistik.

Chen, M., Arato, M., Borghi, L., Nouri, E., & Reinhardt, D. (2018). Beneficial services of arbuscular mycorrhizal fungi: From ecology to application. Frontiers in Plant Science, 9, 1270.

Covre, A.M., Partelli, F.L., Bonomo, R., & Gontijo, I. (2018). Micronutrients in the fruits and leaves of irrigated and non-irrigated coffee plants. Journal of Plant Nutrition, 41(9), 1119-1129.

DaMatta, F.M., Avila, R.T., Cardoso, A.A., Martins, S.C.V., & Ramalho, J.C. (2018). Physiological and agronomic performance of the coffee crop in the context of climate change and global warming: A review. Journal of Agricultural and Food Chemistry, 66(21), 5264-5274.

Evizal, R., Prasmatiwi, F.E., Nurmayasari, I., & Hinrichs, C. (2022). Challenges and opportunities of coffee production in Lampung Province, Indonesia. IOP Conference Series: Earth and Environmental Science, 1027(1), 012028.

Frew, A., Gamper, H.A., Glauser, G., Vanoirbeek, K., & Vasar, M. (2021). Mycorrhizal inoculum type and organic fertilizers drive plant growth promotion. Soil Biology and Biochemistry, 157, 108248.

Genre, A., Lanfranco, L., Perotto, S., & Bonfante, P. (2020). Unique and common traits in mycorrhizal symbioses. Nature Reviews Microbiology, 18(11), 649-660.

Jati, M.K., Santoso, H., & Nugroho, P.A. (2021). Economic analysis of coffee production in Indonesia: Opportunities and challenges. Agricultural Economics Review, 12(1), 45-58.

Kiers, E.T., Duhamel, M., Beesetty, Y., Mensah, J.A., Franken, O., Verbruggen, E., Fellbaum, C.R., Kowalchuk, G.A., Hart, M.M., Bago, A., Palmer, T.M., West, S.A., Vandenkoornhuyse, P., Jansa, J., & Bücking, H. (2016). Reciprocal rewards stabilize cooperation in the mycorrhizal symbiosis. Science, 333(6044), 880-882.

Malhotra, H., Vandana, Sharma, S., & Pandey, R. (2018). Phosphorus nutrition: Plant growth in response to deficiency and excess. In Plant Nutrients and Abiotic Stress Tolerance (pp. 171-190). Singapore: Springer.

Prasetyo, B.H., & Suriadikarta, D.A. (2006). Karakteristik, potensi, dan teknologi pengelolaan tanah Ultisol untuk pengembangan pertanian lahan kering di Indonesia. Jurnal Litbang Pertanian, 25(2), 39-47.

Puschel, D., Janouskova, M., Voříšková, A., Gryndlerova, H., Vosatka, M., & Jansa, J. (2016). Arbuscular mycorrhiza stimulates biological nitrogen fixation in two Medicago spp. through improved phosphorus acquisition. Frontiers in Plant Science, 8, 390.

Rouphael, Y., Franken, P., Schneider, C., Schwarz, D., Giovannetti, M., Agnolucci, M., De Pascale, S., Bonini, P., & Colla, G. (2015). Arbuscular mycorrhizal fungi act as biostimulants in horticultural crops. Scientia Horticulturae, 196, 91-108.

Sharma, A., Shahzad, B., Rehman, A., Bhardwaj, R., Landi, M., & Zheng, B. (2017). Response of phenylpropanoid pathway and the role of polyphenols in plants under abiotic stress. Molecules, 24(13), 2452.

Sharma, S.B., Sayyed, R.Z., Trivedi, M.H., & Gobi, T.A. (2013). Phosphate solubilizing microbes: Sustainable approach for managing phosphorus deficiency in agricultural soils. SpringerPlus, 2, 587.

Wang, M., Jiang, P., & Gao, J. (2017). Effects of arbuscular mycorrhizal fungi on growth and nutrient uptake of tea seedlings. Acta Ecologica Sinica, 37(5), 346-352. Begum, N., Qin, C., Ahanger, M.A., Raza, S., Khan, M.I., Ashraf, M., Ahmed, N., & Zhang, L. (2019). Role of arbuscular mycorrhizal fungi in plant growth regulation: Implications in abiotic stress tolerance. Frontiers in Plant Science, 10, 1068.

Downloads

Published

2026-02-05

How to Cite

Growth Response of Robusta Coffee (Coffea canephora) Cuttings to Arbuscular Mycorrhizal Fungi Dosage Application in Ultisol. (2026). Agricultural Science, 9(2), 328-336. https://doi.org/10.55173/agriscience.v9i2.193